022615 WB SP mCHerry Lysate 3.JPG -> Raw western blot image for lysate samples 022615 WB SP mCHerry Supernatant 3. Files Info 021115_20171219 Correlation_Fluore_vs_Densito_mCherry_50mL_7_days.xlsx -> data points for fluorescence measurements and pixel intensity obtained with FiJI (Fiji Is Just ImageJ).
![fluorescence intensity in zen lite software fluorescence intensity in zen lite software](https://static.cambridge.org/binary/version/id/urn:cambridge.org:id:binary:20201130092754483-0697:S155192952000156X:S155192952000156X_fig6.png)
Supernatant samples were obtained by spinning 1 mL sample at 15000 × g for 10 min and transferring 100 μL from each well to the clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA), followed by fluorescence measurement. Fluorescence was measured at excitation 575/9 nm and emission 608/20 nm.
#Fluorescence intensity in zen lite software pro
Then, 100 μL of each strain was transferred to a clear bottom 96-well plate (Corning Costar, Tewksbury, MA, USA) and fluorescence was measured using an Infinite® M200 PRO plate reader (Tecan, Männedorf, Switzerland).
![fluorescence intensity in zen lite software fluorescence intensity in zen lite software](https://ars.els-cdn.com/content/image/1-s2.0-S2405580821000108-fx3.jpg)
Fluorescence measurements were obtained by growing cc1690 in 50 mL TAP media on a rotary shaker, set to 150 rpm, under constant illumination (50 μmol photons/m2s). Image present in this dataset were obtained by following the steps described in the protocol dx.doi.org/10.17504/protocols.io.kfpctmn. Zen-1118978 Molino, JVD de Carvalho, JCM Mayfield, SP Overview The results present in this dataset were obtained from recombinant Chlamydomonas reinhardtii expressing the fluorescent protein mCherry in different constructs formats, aiming to recombinant protein secretion.